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rabbit anti cep290  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti cep290
    Rabbit Anti Cep290, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+cep290/CEP290+Antibody/pmc12094185-341-41-43
    Average 93 stars, based on 8 article reviews
    rabbit anti cep290 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: A conserved role for centriolar satellites in translation of centrosomal and ciliary proteins
    Article Snippet: .. The following primary antibodies were used for immunofluorescence in vertebrate cells: rabbit anti-hsPCM1 ( ) and goat anti-GFP , both 1 μg/ml, rabbit anti-MIB1 (Sigma-Aldrich), 1:100, rabbit anti-AZI1 (Abcam), 1:250, mouse anti-hsPCM1 (G-6; Santa Cruz Biotechnology), 1:300, rabbit anti-CDK5RAP2 (Merck Millipore), rabbit anti-CEP290 (Novus Biologicals), and rabbit anti-PCNT (Abcam), all 1:400, rabbit anti-OFD1 (Sigma-Aldrich), 1:500, and mouse anti-γ-tubulin (GTU88; Sigma-Aldrich), 1:1,000. .. Secondary antibodies (Jackson ImmunoResearch) were used at 1:100.

    Article Title: Centriolar satellites are sites of translation of centrosomal and ciliary proteins
    Article Snippet: .. The following primary antibodies were used for immunofluorescence in vertebrate cells: rabbit anti-MIB1 (Sigma-Aldrich), 1:100, rabbit anti-AZI1 (Abcam), 1:250, mouse anti-hsPCM1 (G-6, Santa Cruz), rabbit anti-PCM1 , both 1:300, rabbit anti-CDK5RAP2 (Merck Millipore), rabbit anti-CEP290 (Novus Biologicals), rabbit anti-PCNT (Abcam), all 1:400, rabbit anti-OFD1 (Sigma-Aldrich), 1:500, mouse anti-ψ-tubulin (GTU88; Sigma-Aldrich), 1:1000. .. Secondary antibodies (Jackson ImmunoResearch) were used at 1:100.

    Incubation:

    Article Title: Cryo-EM of AKAP350/AKAP9 Reveals Fibrillar Clusters and an Association With DNA.
    Article Snippet: AKAP350 is a massive human scaffolding protein, encoded by AKAP9, that anchors protein kinase A (PKA) to the Golgi apparatus and the centrosome.. AKAP9 is among the most frequently mutated genes in various human cancers, and dysregulation of AKAP350 function is strongly linked to metastasis.. However, the molecular mechanisms underlying these disease processes remain poorly understood due to the challenges of studying its large and unstructured protein sequence.

    Article Title: Cryo-EM of AKAP350 reveals fibrillar clusters and a potential association with DNA
    Article Snippet: .. The membranes were washed with TBST [50 mM Tris, 150 mM NaCl, 1% Tween-20], incubated for 1 hour in blocking buffer (50 mM Tris, 150 mM NaCl, 1% v/v Tween-20, 5% w/v skim milk powder), washed again with TBST, then incubated overnight at 4°C in primary antibody buffer [50 mM Tris, 150 mM NaCl, 1% v/v Tween-20, 5% w/v skim milk powder, 1:1000 mouse anti-FLAG-tag (Sigma Aldrich, F1804), or rabbit anti-CEP290 (Novus, NB 100-86991), or rabbit anti-CDK5RAP2 (Sigma, HPA046529) or rabbit anti-CEP170 (Sigma, HPA042151), or rabbit anti-NCL (CST, #14574), or rabbit anti-NFIB (ThermoFisher Scientific, PA5-%2032), or rabbit anti-β-actin (CST, #4970) primary antibody]. .. The membranes were then washed with TBST and incubated in secondary antibody buffer [50 mM Tris, 150 mM NaCl, 1% v/v Tween-20, 5% w/v skim milk powder, 1:5000 goat anti-rabbit secondary antibody (CST, #7074), or 1:5000 goat anti-mouse secondary antibody (CST, #7076)] for one hour at room temperature before washing with TBST and visualizing with SuperSignal TM West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) and imaging in a Gel Doc XR+ (BioRad).

    Blocking Assay:

    Article Title: Cryo-EM of AKAP350/AKAP9 Reveals Fibrillar Clusters and an Association With DNA.
    Article Snippet: AKAP350 is a massive human scaffolding protein, encoded by AKAP9, that anchors protein kinase A (PKA) to the Golgi apparatus and the centrosome.. AKAP9 is among the most frequently mutated genes in various human cancers, and dysregulation of AKAP350 function is strongly linked to metastasis.. However, the molecular mechanisms underlying these disease processes remain poorly understood due to the challenges of studying its large and unstructured protein sequence.

    Article Title: Cryo-EM of AKAP350 reveals fibrillar clusters and a potential association with DNA
    Article Snippet: .. The membranes were washed with TBST [50 mM Tris, 150 mM NaCl, 1% Tween-20], incubated for 1 hour in blocking buffer (50 mM Tris, 150 mM NaCl, 1% v/v Tween-20, 5% w/v skim milk powder), washed again with TBST, then incubated overnight at 4°C in primary antibody buffer [50 mM Tris, 150 mM NaCl, 1% v/v Tween-20, 5% w/v skim milk powder, 1:1000 mouse anti-FLAG-tag (Sigma Aldrich, F1804), or rabbit anti-CEP290 (Novus, NB 100-86991), or rabbit anti-CDK5RAP2 (Sigma, HPA046529) or rabbit anti-CEP170 (Sigma, HPA042151), or rabbit anti-NCL (CST, #14574), or rabbit anti-NFIB (ThermoFisher Scientific, PA5-%2032), or rabbit anti-β-actin (CST, #4970) primary antibody]. .. The membranes were then washed with TBST and incubated in secondary antibody buffer [50 mM Tris, 150 mM NaCl, 1% v/v Tween-20, 5% w/v skim milk powder, 1:5000 goat anti-rabbit secondary antibody (CST, #7074), or 1:5000 goat anti-mouse secondary antibody (CST, #7076)] for one hour at room temperature before washing with TBST and visualizing with SuperSignal TM West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) and imaging in a Gel Doc XR+ (BioRad).

    Western Blot:

    Article Title: Myocardin-Related Transcription Factor and Serum Response Factor Regulate Cilium Resorption and Ciliogenesis by Both Transcriptional and Local Mechanisms
    Article Snippet: 1 Myocardin-related transcription factor and serum response factor regulate cilium resorption and ciliogenesis by both transcriptional and local mechanisms Pam Speight1#, Matthew Rozycki1#, Katalin Szászi1,2, Shruthi Venugopal1, Michael Kofler1 and András Kapus1,2,3,* Keenan Research Centre for Biomedical Science of the St. Michael’s Hospital1, Dept.. Surgery2 and Dept.. Biochemistry3, University of Toronto, Toronto, ON, Canada M5B 1T8 #These authors contributed equally *Corresponding author: András Kapus Keenan Research Centre for Biomedical Science, Room 621, 209 Victoria Street, Toronto, ON, Canada, M5B 1T8, Tel: 1 416 847 1751; FAX: 1 416 864 5167 E-mail: andras.kapus@unityhealth.to This preprint research paper has not been peer reviewed.

    Article Title: Myocardin-related transcription factor and serum response factor regulate cilium turnover by both transcriptional and local mechanisms
    Article Snippet: MG-132 (Sigma-Aldrich) was used at 20μM. .. Commercial antibodies used were as follows: For western blot analysis; rabbit anti-MRTFA (1:1000, Cell Signaling Technology,14760), rabbit anti-MRTFB (1:1000, Santa Cruz Biotechnology, 98989), mouse anti-MRTFA (1:1000, Santa Cruz Biotechnology, 398675), rabbit anti-HA (1:1,000, Abcam, ab-13834), rabbit anti-SRF (1:1000, Cell Signaling Technology, 5147), mouse anti-c-Myc 9E10 (1:1,000, Santa Cruz Biotechnology, sc-40), mouse anti-GAPDH (1:20,000, Santa Cruz Biotechnology, sc-47724,), rabbit anti-Smad4 (1:1000, Cell Signaling Technology, 9515), rabbit anti-HDAC6 (1:1000, Cell Signaling Technology, 7612), mouse anti-HEF1 (1:1000, Cell Signaling Technology, 4044), rabbit anti-CEP290 (1:500, Novus Biologicals, NB100-86991), mouse anti-Aurora A (1:1000,Thermo Fisher Scientific/Invitrogen, 45-8900), rabbit anti-Tubulin (1:10,000, Cell Signaling Technology, 2128), mouse anti- β-actin ( 1:10,000, Sigma-Aldrich, A1978), rabbit anti-Antizyme1 (1:250, Enzo Life Sciences, BML-PW8885), rabbit anti-AURKAIP1 (1:500, MyBiosource, MBS2516763), rabbit anti-C6orf134 (1:500, Abcam, ab58742), mouse anti-Acetylated Tubulin (1:500, Sigma-Aldrich, T7451). .. For immunofluorescence; mouse anti-Acetylated Tubulin (1:200, Abcam, ab24610), rabbit anti- Arl13b (1:100, Proteintech, 17711-1-AP), rabbit anti-Nup98 (1:1000, Santa Cruza Biotechnology, sc-74578); rabbit anti-Histone H1 FL-219 (1:100, Santa Cruz, Biotechnology, sc-10806), mouse anti-CEP290 (1:50, Santa Cruz Biotechnology, sc-390637), rabbit anti-gamma Tubulin (1:100, Abcam, ab16504), mouse anti-gamma Tubulin (1:100, Abcam, ab27074), rabbit anti-SRF (1:1000, Cell Signaling Technology, 5147), rabbit anti-MRTFA (1:100, Cell Signaling Technology, 14760), rabbit anti-MRTFB (1:50, Santa Cruz Biotechnology, 98989), mouse anti-MRTFA (1:50, Santa Cruz Biotechnology, sc-398675), goat anti-MRTFB (1:50, Santa Cruz Biotechnology, sc-47282), rabbit anti-MRTFA (1:50, Santa Cruz Biotechnology,sc-32909) and rabbit-anti-Ki67 (1:100, Abcam, ab15580).



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